TABLE 2.

Primers used in PCR amplification of Tat secretion system genes and putative Tat-secreted genes

Primer	Target	Sequence
tatCshortL	Internal tatC	5′-CTTCAACCTGTTCTCGCGTC-3′
tatCshortR		5′-CAACAGCTGCGACATCACAT-3′
TATORFL	tatABC	5′-GCTGCCTTTTCTTTTGGATG-3′
TATORFR		5′-TATCTCAACGACAGCAAGCC-3′
Rs04798L	RSp1521	5′-TACATTGACTGATGTCGCGG-3′
Rs04798R		5′-GTAATCGCCGTCGAAGTGA-3′
Rs02131L	RSp1575	5′-ATTGCCGATATCAAGAAGCG-3′
Rs02131R		5′-AACCACTTTGTCTGCAGCG-3′
Rs04028L	RSc1651	5′-GTCGGAGGACATCATGGAAC-3′
Rs04028R		5′-CTACGCAAGCTGTGGCTG-3′
PehCKRKKFor	pehC	5′-GCTCGCATTCGCCAGCGAAGAAGGCATTCGTCGTCTGGTC-3′
PehCKRKKRev		5′-GACCAGACGACGAATGCCTTCTTCGCTGGCGAATGCGAGC-3′