Electrophoretic mobility of components and assembled trifunctional complexes on nondenaturing gel. Lane 1, Scaf3 alone; lane 2, 9Gc alone; lane 3, 6At alone; lane 4, chimeric cellulosome containing Scaf3, 6At, and 9Gc; lane 5, Scaf4 alone; lane 6, chimeric cellulosome containing Scaf4, 6At, and 9Gc. In each lane, equimolar concentrations (10 μmol/liter) of the indicated proteins were used. Similar-quality gels were obtained for all of the complexes used in this study.