FIG. 4.

Kinetic studies of Avicel hydrolysis by enzyme pairs in the free state or complexed with Scaf3 or Scaf4. The proteins were mixed in stoichiometric amounts, and the status of the protein mixture was verified by nondenaturing PAGE (data not shown) prior to addition of the substrate. The panels indicate the selected enzyme pair as follows: A, 6At and 5Ac; B, 6At and 9Ec; C, 6At and 48Fc; D, 6At and 9Gc; and E, 6At and 9Mc. Curves are labeled as follows: free enzyme pair, ▾; enzyme pair complexed to Scaf3, •; enzyme pair complexed to Scaf4, ○. The data are the means of four independent experiments, and the bars indicate the standard deviations.