FIG. 5.

Avicelase activities of 6At plus 9Gc (A) and 6At plus 5Ac (B) bound jointly or individually to Scaf3 and Scaf4. The designated proteins were mixed in stoichiometric amounts, and the status of the protein mixture was verified by nondenaturing PAGE (data not shown) prior to addition of the substrate. White bars, soluble sugars released by single-enzyme complexes; gray bars, soluble sugars released by Scaf3- and Scaf4-based complexes; black bars, calculated (calc.) sum of soluble sugars released by single-enzyme complexes individually. The data are the means of four independent experiments, and the bars indicate the standard deviations.