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. 2007 Apr 27;73(12):3822–3832. doi: 10.1128/AEM.00398-07

FIG. 9.

FIG. 9.

Kinetic studies of Avicel hydrolysis by trifunctional complexes versus controls. The designated proteins were mixed in stoichiometric amounts, and the status of the protein mixture was verified by nondenaturing PAGE (data not shown) prior to addition of the substrate. The panels indicate the selected enzymes as follows: panel A, 6At, 9Gf, and 48Fc; and panel B, 6At, 9Gf, and 9Ec. Curves are labeled as follows: free-enzyme systems (▾) composed of 6At, 9Gf, and 48Fc (A) and 6At, 9Gf, and 9Ec (B); bifunctional complexes (•) Scaf6-(48Fc/9Gf) (A) and Scaf6-(9Ec/9Gf) (B); trifunctional complexes (○) Scaf6-(6At/48Fc/9Gf) (A) and Scaf6-(6At/9Ec/9Gf) (B). Dashed line, calculated soluble sugars released by Scaf6-(48Fc/9Gf) plus 6At (A) and Scaf6-(9Ec/9Gf) plus 6At (B). The data are the means of four independent experiments, and the bars indicate the standard deviations.