FIG. 2.

B. subtilis strain natto. (A) The soybean surface was covered with strain natto cells. The LB plate surface was streaked with natto and incubated at 37°C. (B) Growth of strain natto cells on boiled soybean minimal medium. Viable cells in culture broth were determined by measuring colony counts after spreading on LB medium. (C) Genetic organization of the yes cluster. (D) Expression of 16S rRNA, YesW, and YesX genes in strain natto cells grown on soybeans. Each gene was amplified by using total RNAs after treatment with (+RT) and without (−RT) reverse transcriptase.