TABLE 3.
Effects of metal ions on YesW and YesX activity
| Pretreatmenta | Metal(s) added (1 mM concn) | Activity (%)b
|
|
|---|---|---|---|
| YesW | YesX | ||
| CaCl2 (1 mM) | CaCl2 | 100 | 100 |
| None | 0 | 7 | |
| EGTA (10 mM) | None | 0 | 0 |
| EDTA (10 mM) | None | 0 | 0 |
| AlCl3 | 0 | 16 | |
| MgCl2 | 4 | 0 | |
| MnCl2 | 134 | 661 | |
| CaCl2 | 54 | 89 | |
| CoCl2 | 54 | 111 | |
| ZnCl2 | 21 | 579 | |
a
YesW and YesX were treated with 10 mM EDTA or EGTA and then dialyzed against 20 mM of Tris-HCl (pH 7.5) containing 0.2 M NaCl. The activity of these enzymes pretreated with EDTA was determined in the presence of different metal ions at the indicated concentration.
b
The activity of chelator-treated enzymes in the presence of CaCl2 is taken as 100%.