TABLE 1.
Plasmids and strains used in the present study
| Plasmid or strain | Relevant properties or genetic marker | Source or reference(s) |
|---|---|---|
| Plasmids | ||
| pACYCDuet-1 | P15A(pACYC184), Cmr | Novagen |
| pCOLADuet-1 | ColA, Kmr | Novagen |
| pETDuet-1 | ColE1(pBR322), Ampr | Novagen |
| pCDFDuet-1 | CloDF13, Strr | Novagen |
| pTrcHis2 | pBR322, Ampr | Invitrogen |
| pC-Pc4cl2 | pCDFDuet-1 carrying 4cl-2 from Petroselinum crispum | 25 |
| pE-Phchs-Phchi | pETDuet-1 carrying chs, chi from Petunia hybrida | 23, 25 |
| pE-Phchs-Mschi | pETDuet-1 carrying chs from P. hybrida, chi from Medicago sativa | This study |
| pR-accD | pACYCDuet-1 carrying accD from P. luminescens | |
| yE-accA | YEplac195 carrying accA from P. luminescens | This study |
| yE-accA-T7accD | yE-accA carrying T7lac-accD fusion | This study |
| pA-accBC | pACYCDuet-1 carrying accBC from P. luminescens | This study |
| pA-Placc | pACYCDuet-1 carrying accABCD from P. luminescens | This study |
| pT-PlbirA | pTrcHis2 carrying birA from P. luminescens | This study |
| pT-EcbirA | pTrcHis2 carrying birA from E. coli K-12 | This study |
| pT-ChbirA | pTrcHis2 carrying chimeric birA | This study |
| pC-PlbirA | pCOLADuet-1 carrying birA from P. luminescens | This study |
| pC-EcbirA | pCOLADuet-1 carrying birA from E. coli K-12 | This study |
| pC-ChbirA | pCOLADuet-1 carrying chimeric birA | This study |
| pC-ackA-pta | pCOLADuet-1 carrying ackA, pta from E. coli K-12 | This study |
| pC-acs | pCOLADuet-1 carrying acs from E. coli K-12 | This study |
| Strains | ||
| E. coli BL21Star | F−ompT hsdSB (rB− mB−) gal dcm rne131 (DE3) | Invitrogen |
| E. coli BM4062 | araD139([]) DE(argF-lac)169 LAM TP(bioF-lacZ)501 flhD5301 DE(fruK-yeiR)725(fruA25)relA1 rpsL150(strR) rbsR22 birA85 DE(fimB-fimE)632(::IS1) deoC1 | The E. coli Genetic Resource Center |
| P. luminescens | Type strain | ATCC |
| E. coli K-12 | Type strain | ATCC |
| E1 | BL21Star(pC-Pc4cl2, pE-Phchs-Phchi) | This study |
| E2 | BL21Star(pC-Pc4cl2, pE-Phchs-Mschi) | This study |
| E1A/E2A | E1(pA-Placc)/E2(pA-Placc) | This study |
| E1ABp/E2ABp | E1A(pC-PlbirA)/E2A(pC-PlbirA) | This study |
| E1ABe/E2ABe | E1A(pC-EcbirA)/E2A(pC-EcbirA) | This study |
| E1ABc/E2ABc | E1A(pC-ChbirA)/E2A(pC-ChbirA) | This study |
| E1AAP/E2AAP | E1A(pC-ackA-pta)/E2A(pC-ackA-pta) | This study |
| E1AA/E2AA | E1A(pC-acs)/E2A(pC-acs) | This study |