TABLE 3.
Flavanone production from engineered strains with PlACC coexpression with BirAPl (E1ABp/E2ABp), BirAEc (E1ABe/E2ABe), or BirACh (E1ABc/E2ABc)a
| Strain and flavanone | BirAPl
|
BirAEc
|
BirACh
|
ΔPp | ΔPe | ΔPc | |||
|---|---|---|---|---|---|---|---|---|---|
| Pp | G | Pe | G | Pc | G | ||||
| E1A | |||||||||
| Pinocembrin | 2 | 3 | 1 | 3 | 2 ± 1 | 3 | 100 | 0 | 100 |
| Naringenin | 5 | 3 | 3 | 3 | 5 | 3 | 67 | 0 | 67 |
| Eriodictyol | 0.2 | 4 | 0.1 | 4 | 0.1 | 4 | 100 | 0 | 0 |
| E2A | |||||||||
| Pinocembrin | 367 ± 1 | 3 | 226 ± 17 | 3 | 355 ± 2 | 3 | 1,166 | 679 | 1,124 |
| Naringenin | 69 ± 2 | 3 | 61 ± 3 | 3 | 69 ± 2 | 3 | 64 | 45 | 64 |
| Eriodictyol | 50 ± 1 | 4 | 29 ± 1 | 3 | 30 | 3 | 355 | 164 | 173 |
a
Pp, Pe, Pc, production (mg/liter) from E1ABp/E2ABp, E1ABe/E2ABe, and E1ABc/E2ABc, respectively; G, growth level (A600); ΔPp, production increase (%) of E1ABp and E2ABp relative to E1 and E2, respectively; ΔPe, production increase (%) of E1ABe and E2ABe relative to E1 and E2, respectively; ΔPc, production increase (%) of E1ABc and E2ABc relative to E1 and E2, respectively.