TABLE 5.
Flavanone production from engineered strains with PlACC coexpression with AckA and Pta (E1AAP/E2AAP) or Acs (E1AA/E2AA)a
| Strain and flavanone | AckA Pta
|
Acs
|
ΔPap | ΔPaa | ||
|---|---|---|---|---|---|---|
| Pap | G | Paa | G | |||
| E1A | ||||||
| Pinocembrin | 3 | 3 | 4 | 3 | 200 | 300 |
| Naringenin | 8 ± 1 | 4 | 8 | 4 | 167 | 167 |
| Eriodictyol | 2 | 3 | 3 | 3 | 1,900 | 2,900 |
| E2A | ||||||
| Pinocembrin | 405 ± 11 | 3 | 429 ± 3 | 3 | 1,297 | 1,379 |
| Naringenin | 104 | 3 | 119 | 3 | 148 | 183 |
| Eriodictyol | 45 ± 4 | 3 | 52 ± 1 | 3 | 309 | 373 |
a
Pap, Paa, production (mg/liter) from E1AAP/E2AAP and E1AA/E2AA, respectively; G, growth level (A600); ΔPap, production increase (%) of E1AAP and E2AAP relative to E1 and E2, respectively; ΔPaa, production increase (%) of E1AA and E2AA relative to E1 and E2, respectively. Cultures were grown in minimal media supplemented with 2 g/liter acetate in addition to glucose.