TABLE 1.
Sample group | No. of samples | Source(s) or reference | Pooled for extraction | NA extraction method | No. (%) tested PCR positive for the indicated speciese
|
|||
---|---|---|---|---|---|---|---|---|
M. haemofelis | “Ca. Mycoplasma haemominutum” | “Ca. Mycoplasma turicensis” | M. coccoides | |||||
Ticks from animals | ||||||||
Ixodes sp. | 71 | 39 cats | 1-3 | DNeasy tissue kit | 0 | 2 (2.8) | 0 | ND |
110 | 66 dogs | 1-4 | DNeasy tissue kit | 0 | 0 | 0 | ND | |
Rhipicephalus sp. | ||||||||
Rhipicephalus sp. | 26 | Miscellaneous | DNeasy tissue kit | 0 | 0 | 0 | ND | |
R. sanguineus | 23 | 3 | QIAamp DNA minikit | 0 | 0 | 1 (4.3)d | ND | |
R. turanicus | 18 | 3 | QIAamp DNA minikit | 0 | 0 | 0 | ND | |
Ticks from vegetation | 1,950 | Vegetation | 10 | MagNa Pure | 0 | 0 | 0 | ND |
Fleas | ||||||||
C. felis | 73 | 17 cats | 1-5 | DNeasy tissue kit | 0 | 2 (2.7) | 0 | ND |
C. canis | 4 | 4 cats | DNeasy tissue kit | 0 | 0 | 0 | ND | |
Rodents | ||||||||
A. terrestris | 186 | Free-living rodentsb,c | MagNa Pure | 0 | 0 | 0 | 0 | |
Apodemus sp. | 45 | Free-living rodents | MagNa Pure | 0 | 0 | 0 | 24 (53) | |
M. glareolus | 11 | Free-living rodentsc | MagNa Pure | 0 | 0 | 0 | 1 (9.1) | |
Microtus sp. | 7 | Free-living rodentsc | MagNa Pure | 0 | 0 | 0 | 0 | |
M. domesticus | 7 | Free-living rodents | MagNa Pure | 0 | 0 | 0 | 0 |
The table shows the species, number, source, and extraction methods used for ticks, fleas, and rodents collected from throughout Switzerland and the numbers and percentages that tested PCR positive for M. haemofelis, “Ca. Mycoplasma haemominutum,” “Ca. Mycoplasma turicensis,” and M. coccoides.
Reference 20 (Schwarzenbach et al., 2004).
Reference 23 (Stieger et al., 2002).
Collected indoors from a dog-keeping household or directly from a dog.
ND, not determined.