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. 2007 Apr 27;73(12):4001–4010. doi: 10.1128/AEM.02323-06

TABLE 3.

Primers used for genome walking and secY, secY-map, and uvrB-degV amplification and sequencing

Genetic locus Method Primer Sequence (5′-3′)
uvrB Genome walking MFD32g1 TAGGAATTAAAGTAGCTTATCTTCATAGTG
MFD32gN1 CTGGTGTTTATGATTGTTTAGTTGGA
secY Genome walking MFD9g1 TTGTAAGATGACGATCAGAATTAGGA
MFD9g1N GCAAAGATGTAGCGGAACATTTGTC
PCR FD9r TTTGCTTTCATATCTTGTRTCG
FD9f2L GTTTTAGCTAAAGGTGATTTAAC
Nested PCR FD9r2L TAAAAGACTAGTCCCRCCAAAAG
FD9f3L AATAAGGTAGTTTTATATGACAAG
Sequencing FD9ri CTATTTATAGCGTAATTAATAGG
secY-map PCR FD9f5 CAAAAAATTACTTTTGGCGGGAC
MAPr1 TGCTCAAAATGAGCGCTTAAAC
Nested PCR FD9f6 GTCGCTTTAGAATCGACACA
MAPr2 TCGGAAGTAACAGCAGTCCA
uvrB-degV PCR UVRBf1 GAAGGTCTAGATTTGCCTGAAGT
DEGVr4 CTCCATTTTTGTTAACCTGT
Nested PCR UVRBf3 TTAATCCAAACTATCGGAAGA
DEGVr3 CCTTTTGTTTTGTTTAAACGTCC
Sequencing DegVf1 GCTTCGACATCAACTAGTTG