TABLE 3.
Primers used for genome walking and secY, secY-map, and uvrB-degV amplification and sequencing
| Genetic locus | Method | Primer | Sequence (5′-3′) |
|---|---|---|---|
| uvrB | Genome walking | MFD32g1 | TAGGAATTAAAGTAGCTTATCTTCATAGTG |
| MFD32gN1 | CTGGTGTTTATGATTGTTTAGTTGGA | ||
| secY | Genome walking | MFD9g1 | TTGTAAGATGACGATCAGAATTAGGA |
| MFD9g1N | GCAAAGATGTAGCGGAACATTTGTC | ||
| PCR | FD9r | TTTGCTTTCATATCTTGTRTCG | |
| FD9f2L | GTTTTAGCTAAAGGTGATTTAAC | ||
| Nested PCR | FD9r2L | TAAAAGACTAGTCCCRCCAAAAG | |
| FD9f3L | AATAAGGTAGTTTTATATGACAAG | ||
| Sequencing | FD9ri | CTATTTATAGCGTAATTAATAGG | |
| secY-map | PCR | FD9f5 | CAAAAAATTACTTTTGGCGGGAC |
| MAPr1 | TGCTCAAAATGAGCGCTTAAAC | ||
| Nested PCR | FD9f6 | GTCGCTTTAGAATCGACACA | |
| MAPr2 | TCGGAAGTAACAGCAGTCCA | ||
| uvrB-degV | PCR | UVRBf1 | GAAGGTCTAGATTTGCCTGAAGT |
| DEGVr4 | CTCCATTTTTGTTAACCTGT | ||
| Nested PCR | UVRBf3 | TTAATCCAAACTATCGGAAGA | |
| DEGVr3 | CCTTTTGTTTTGTTTAAACGTCC | ||
| Sequencing | DegVf1 | GCTTCGACATCAACTAGTTG |