FIG. 2.

Maps of treY (A) and otsA (B) loci in R. leguminosarum bv. trifolii strain NZP561. The locations and orientations of open reading frames with homologs in databases are shown by arrows. The identification tags of corresponding genes in R. leguminosarum bv. viciae strain 3841 (58) are shown in parentheses. The positions of the transposon insertions in the EZTn5 mutants are indicated by inverted triangles. The locations of PCR products used for the construction of pFUS2 IDM and CMD strains or used as probes for library screening are shown by rectangles. E, EcoRI sites.