TABLE 2.
Primers used in this study
| Primer | Sequence (5′ to 3′)a | Use |
|---|---|---|
| treYF | CACGACACCAAACGCGGTGA | treY probe |
| treYR | CGACGGTTGTCGGGATCAAC | treY probe |
| otsAF | TATGGATGGGCTGGTCGGGAAAGT | otsA probe |
| otsAR | TGCAGGCGGCCAGGAATGTG | otsA probe |
| treYIDMR | AAATTTGGATCCGTCGCCATAGGTGCGGTAGA | IDM |
| treYIDML | AAATTTAAGCTTGGCCGGACACCTATATCGTC | IDM |
| otsAIDMR | AAATTTGGATCCCGAATAATCGAGGCGGTCGA | IDM |
| otsAIDML | AAATTTAAGCTTCGGCTTCTTCCTGCACATTC | IDM |
| treYCMDR | AAATTTGGATCCATCGAAGGTCATGCCGTTGC | CMD |
| treYCMDL | AAATTTAAGCTTCTGTCACCGCTGATGCTGAA | CMD |
| otsACMDR | AAATTTGGATCCGGCAGCGGCGCTCCCATCTT | CMD |
| otsACMDL | AAATTTAAGCTTAGCGGTCGGCCTGGCACATC | CMD |
| lacZ | GCTATTACGCCAGCTGGCGA | Sequencing |
| KAN-FP1 | ACCTACAACAAAGCTCTCATCAACC | Sequencing |
| KAN-RP1 | GCAATGTAACATCAGAGATTTTGAG | Sequencing |
a
Restriction sites incorporated into primers are underlined.