TABLE 3.
Trehalose accumulation by early-stationary-phase cultures of strain NZP561 and trehalose biosynthesis mutants
| Strain (genotype) | Trehalose concn (μg per mg protein)a
|
|
|---|---|---|
| 42 h | 46 h | |
| NZP561 (wild type) | 111.7 ± 3.5 (4) | 140.2 ± 4.4 (1) |
| HM1 (otsA) | 93.8 ± 2.6 (3) | 120.4 ± 1.9 (1) |
| HM2 (treY) | 101.8 ± 2.9 (2) | 134.2 ± 1.9 (1) |
| HM3 (otsA) | 91.17 ± 2.9 (3) | 130.4 ± 2.1 (1) |
| HM5 (otsA treY) | 0 (3) | 0 (1) |
| TH1 (otsA treY) | 0 (3) | 0 (1) |
a
The values are means ± standard errors. The number of independent assays for each strain is shown in parentheses. For each assay, two samples were analyzed in triplicate.