TABLE 5.
β-Galactosidase specific activities of strain NZP561 and the CMD strains HM12 (treY::lacZ) and TH3 (otsA::lacZ) throughout the growth cycle
| Growth cycle
|
β-Galactosidase sp acta
|
|||
|---|---|---|---|---|
| Time (h) | Phase | NZP561 | HM12 (treY::lacZ) | TH3 (otsA::lacZ) |
| 18 | Exponential | 38.0 ± 5.5 (4) | 164.1 ± 11.1 (4) | 94.5 ± 7.3 (3) |
| 24 | Exponential | 21.2 ± 0.8 (4) | 161.2 ± 10.4 (4) | 97.0 ± 4.6 (6) |
| 42 | Early stationary | 24.7 ± 2.6 (3) | 163.1 ± 11.2 (3) | 102.8 ± 3.2 (4) |
| 47 | Early stationary | 20.9 ± 1.6 (3) | 159.1 ± 10.3 (3) | 92.4 ± 1.2 (4) |
| 69 | Stationary | 25.1 ± 1.6 (6) | 155.3 ± 9.0 (5) | 77.6 ± 3.7 (6) |
| 90 | Stationary | 14.0 ± 2.9 (3) | 110.1 ± 72 (3) | 55.7 ± 4.9 (4) |
a
Expressed in nanomoles of O-nitrophenol released per minute per milligram of protein. Each sample was assayed in triplicate. The number of independent samples assayed for each strain is shown in parentheses.