TABLE 6.
Competition for T. repens nodule occupancy between strain NZP561 and double trehalose biosynthesis mutants at saturating inoculum density
| Strain mixture | Inoculum ratio (NZP561/mutant) | Total no. of nodules screeneda | Expected no. of mutant nodules (%) | Observed no. of mutant nodules (%) | P valueb |
|---|---|---|---|---|---|
| NZP561 + HM5 (106 cells/plant) | 1:1 | 67 | 50 | 28.3 | 0.0002 |
| 1:3 | 57 | 75 | 43.8 | 0 | |
| 3:1 | 65 | 25 | 9.2 | 0.002 | |
| NZP561 + TH1 (106 cells/plant) | 1:1 | 66 | 50 | 33.3 | 0.009 |
| 1:3 | 25 | 75 | 52 | 0.018 | |
| NZP561 + TH1 (2 × 106 cells/plant) | 1:1 | 71 | 50 | 15.5 | 0 |
| 1:3 | 20 | 75 | 50 | 0.02 |
a
Nodules that contained both strains were excluded from the analysis.
b
A two-sided bionomial test was used to compare the observed outcomes with the expected outcomes. P values indicate the probability that the observed values differ from the expected outcomes by chance.