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. 2007 Apr 20;73(12):4040–4047. doi: 10.1128/AEM.00161-07

TABLE 3.

Effect of changing TaqMan real-time PCR cycling conditions on CT values for the detection of P. ramorum DNAa

Cycling conditions Approximate run time for 40 cycles (min) Mean CT value Approximate time to positive result (min)
15 s denature, 60 s anneal 70 19.37 39
5 s denature, 60 s anneal 63 19.93 37
5 s denature, 30 s anneal 43 24.03 30
5 s denature, 25 s anneal 40 26.98 30
5 s denature, 20 s anneal 37 29.38 30
a

Approximately 10 ng DNA per reaction mixture.