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Effect of changing TaqMan real-time PCR cycling conditions on CT values for the detection of P. ramorum DNAa
| Cycling conditions | Approximate run time for 40 cycles (min) | Mean CT value | Approximate time to positive result (min) |
|---|---|---|---|
| 15 s denature, 60 s anneal | 70 | 19.37 | 39 |
| 5 s denature, 60 s anneal | 63 | 19.93 | 37 |
| 5 s denature, 30 s anneal | 43 | 24.03 | 30 |
| 5 s denature, 25 s anneal | 40 | 26.98 | 30 |
| 5 s denature, 20 s anneal | 37 | 29.38 | 30 |
Approximately 10 ng DNA per reaction mixture.
