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. 2007 May 4;73(13):4142–4151. doi: 10.1128/AEM.02729-06

FIG. 2.

FIG. 2.

SDS-PAGE and isoelectric focusing gel of zinc-containing metalloprotease preparations from E. sakazakii strain 51329. (A) SDS-PAGE. Lanes: 1, molecular mass markers (values at left are in kilodaltons); 2, crude metalloprotease preparation (1.5 μg); 3, purified metalloprotease preparation (black arrow, 0.05 μg). Gel was stained with Coomassie brilliant blue R. (B) Analytical thin-layer isoelectric focusing. (a) Stained gel. Lanes: 1, pI markers; 2, purified metalloprotease preparation (0.4 μg). The gel was stained with Coomassie brilliant blue R. (b) Zymogram analysis of isoelectric focusing gel using an overlay of casein in 1% Noble agar.