TABLE 2.

Inactivation of E. sakazakii protease

Treatment	Residual activity (%)
None	100
ortho-Phenanthroline, 0.1 mM	39
Zincov, 0.1 mM	39
N-ethylmaleimide, 0.1 mM	100
Phenymethylsulfonylfluoride, 0.1 mM	100
Trypsin inhibitor, 10 μg	100
65°C, 15 min	68
100°C, 15 min	0
pH 4-10 at 4°C, 16 h	100