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. 2007 May 4;73(13):4368–4372. doi: 10.1128/AEM.00086-07

TABLE 1.

JS614 proteins expressed in response to VC, ethene, and epoxyethane with statistical relevance (P < 0.05), identified by using MALDI-TOF MS PMF analysisl

Excised gel sectiona Growth substrateb Significant Mascot hitsc Predicted mass (kDa)d Observed mass (kDa)e Mowse scoref E valueg % Coverageh NBMi MM/TPMSj Gene no.k
1 Epoxyethane FAD-dependent pyridine nucleotide-disulfide oxidoreductase 60.47 74 142 1.6E−08 37 61 14/49 4827
1 VC FAD-dependent pyridine nucleotide-disulfide oxidoreductase 60.47 79 101 2.5E−04 28 58 12/55 4827
1 Ethene FAD-dependent pyridine nucleotide-disulfide oxidoreductase 60.47 73 141 7.5E−07 30 60 14/41 4827
2 Epoxyethane Probable alkene monooxygenase alpha subunit 57.78 62 178 1.5E−06 44 59 19/70 4807
2 VC Probable alkene monooxygenase alpha subunit 57.78 65 142 1.5E−08 36 75 19/66 4807
2 Ethene Probable alkene monooxygenase alpha subunit 57.78 65 126 9.0E−03 33 56 13/56 4807
3 Epoxyethane Alcohol/acetaldehyde dehydrogenase 51.78 54 138 9.5E−08 46 60 15/72 4833
3 VC Alcohol/acetaldehyde dehydrogenase 51.78 55 119 3.3E−06 41 61 14/77 4833
3 Ethene Alcohol/acetaldehyde dehydrogenase 51.78 53 115 1.2E−04 41 62 14/68 4833
3 Epoxyethane Bifunctional protein: alcohol/acetaldehyde dehydrogenase 50.02 54 149 3.0E−06 42 59 14/52 4822
3 VC Bifunctional protein: alcohol/acetaldehyde dehydrogenase 50.02 55 129 5.7E−03 40 59 14/66 4822
3 Ethene Bifunctional protein: alcohol/acetaldehyde dehydrogenase 50.02 53 112 6.5E−04 35 64 14/61 4822
4 Epoxyethane Epoxyalkane:coenzyme M transferase 40.68 45 166 1.9E−08 50 60 13/40 4810
4 VC Epoxyalkane:coenzyme M transferase 40.68 46 163 2.4E−08 49 60 13/46 4810
4 Ethene Epoxyalkane:coenzyme M transferase 40.68 45 150 2.1E−04 45 62 13/47 4810
5 Epoxyethane Short-chain dehydrogenase/reductase 25.85 31 140 3.4E−07 51 63 11/46 4814
5 VC Short-chain dehydrogenase/reductase 25.85 33 158 1.5E−09 59 64 12/40 4814
5 Ethene Short-chain dehydrogenase/reductase 25.85 31 145 2.0E−06 53 61 10/35 4814
5 Epoxyethane Short-chain dehydrogenase/reductase 26.20 31 149 1.2E−07 59 61 12/45 4841
5 VC Short-chain dehydrogenase/reductase 26.20 33 170 1.2E−09 61 64 12/40 4841
5 Ethene Short-chain dehydrogenase/reductase 26.20 31 163 1.5E−09 60 63 12/38 4841
a

Gel sections were excised as shown in Fig. 1.

b

Growth conditions and methods are described in reference 18.

c

Most significant Mascot hits to current gene annotations in the NCBInr database. FAD, flavin adenine dinucleotide.

d

Theoretical mass reported by Mascot.

e

Estimated from results of SDS-PAGE (Fig. 1).

f

Mowse score reported by Mascot.

g

E value reported by Mascot.

h

Percent coverage of amino acids to matched peptides reported by Mascot.

iMowse score of the next best Mascot match (NBM) not associated with JS614.

j

MM, mass matches; TPMS, total number of peptide masses searched.

k

Gene number in finished JS614 genome sequence corresponding to Mascot hit. All NBMs, with the exception of one unknown lipoprotein match to Noc4827 (Mowse score, 80), were not significant. A putative translation elongation factor (encoded by Noc3922; Mowse score, 162) was the only peptide consistently identified in acetate-grown cells (Fig. 1).

l

All proteins shown were found in at least two independent analyses, and the results reported are averaged values.