TABLE 2.
Oligonucleotides used for RT-PCR
| Oligonucleotide (5′ to 3′) | Sequence (5′ to 3′) | Gene no.a | Expected product size (bp) | Reference |
|---|---|---|---|---|
| CoM-F3 | GCTCTCAAGATGTGCTTCTGCCAACCA | Noc4810 | 831 | 18 |
| CoM-R3 | CGGTGCGTCCGACCTCGTAGTTCAG | Noc4810 | 831 | 18 |
| etnC_F | CTTGAAACCGTCCACGAGAAGAG | Noc4807 | 1,481 | 18 |
| etnC_R | AGCGGGTCCTTGATCTCGTACTT | Noc4807 | 1,481 | 18 |
| scdehd_F | GCCATAGGCGTACTTGACCTG | Noc4814 | 560 | Present study |
| scdehd_R | ATGGGGTACTTCACGAGTGTCT | Noc4814 | 560 | Present study |
| carb_F | GGAGTACGGCTCCTTCTACCA | Noc4827 | 832 | Present study |
| carb_R | GTTGAGGAACAACTCATTCATCTC | Noc4827 | 832 | Present study |
| dehy_F | ATCACGTCAATACTGGGGATGT | Noc4833 | 703 | Present study |
| dehy_R | AGGACAAGGTCGTCAAGATCAA | Noc4833 | 703 | Present study |
| 27f | AGAGTTTGATC(C/A)TGGCTCAG | N/A | 1,482 | 16 |
| 1492r | TACGG(C/T)TACCTTGTTACGACTT | N/A | 1,482 | 16 |
| rpoB_F | GCTTCGGGTTGAAGTAGTAGTTGT | Noc0711 | 802 | 17 |
| rpoB_R | GCAAAGATCACCGAACCTCTC | Noc0711 | 802 | 17 |
a
Gene from the JS614 genome sequence that was used to design the oligonucleotide. N/A, not applicable.