FIG. 2.

Bacterial T-RFLP fingerprinting of density-resolved RNA from representative ¹³C-labeled and unlabeled fractions of sludges MP (A), TP (B), MBF (C), and JET (D). Electropherograms of sludges MP, TP, and JET were generated from gradient fractions of RNA from the second ¹³C-palmitate incubation as the template, whereas sludge MBF was generated from gradient fractions of RNA from the first ¹³C-palmitate incubation as the template. Dashed boxes show T-RFLP fingerprints of density-resolved unlabeled control RNA. CsTFA BDs (g·ml⁻¹) of gradient fractions are given in brackets. The numbers at the T-RF peaks indicate the T-RF lengths. Representing phylotypes of T-RFs identified by clone analysis are indicated in parenthesis.