TABLE 1.
Specificity of PCR amplification of cnorB genes from control denitrifier and field isolatesa
| Strain | Denitrification | cnorBP | cnorBB | Source |
|---|---|---|---|---|
| Azoarcus tolulyticus ATCC 51758 | + | − | − | ATCCb |
| Ochrobactrum anthropi ATCC 49687 | + | − | − | Oxoidc |
| Pseudomonas stutzeri ATCC 17588 | + | − | − | ATCC |
| Pseudomonas sp. strain G-179 | + | − | − | UBCd |
| Achromobacter cycloclastes ATCC 21921 | + | − | − | ATCC |
| Paracoccus denitrificans ATCC 17741 | + | − | − | ATCC |
| Bacillus azotoformans ATCC 29788 | + | − | − | ATCC |
| Paracoccus denitrificans ATCC 19367 | + | − | − | ATCC |
| Paracoccus pantotrophus DSM65 | + | − | − | DSMZe |
| Pseudomonas stutzeri JM300 | + | − | − | UBC |
| Thauera aromatica DSM14793 | + | − | − | DSMZ |
| Pseudomonas stutzeri ATCC 14405 | + | − | − | ATCC |
| Bradyrhizobium japonicum USDA110 | + | − | + | USDA-ARSf |
| Alcaligenes faecalis subsp. faecalis ATCC 8750 | + | − | − | Oxoid |
| Cupriavidus necator DSM428 | + | − | − | DSMZ |
| Escherichia coli ATCC 29425 | − | − | − | ATCC |
| Alcaligenes faecalis ATCC 35655 | − | − | − | Oxoid |
| Enterobacter cloacae ATCC 23355 | − | − | − | Oxoid |
| Ochrobactrum anthropi ATCC 49187 | − | − | − | ATCC |
| Pseudomonas migulae PD1 | + | − | − | PRCg |
| Pseudomonas mandelii PD2 | + | + | − | PRC |
| Pseudomonas sp. strain PD3 | + | − | − | PRC |
| Pseudomonas brassicacearum PD4 | + | − | − | PRC |
| Pseudomonas brassicacearum PD5 | + | − | − | PRC |
| Pseudomonas sp. strain PD6 | + | + | − | PRC |
| Achromobacter sp. strain PD7 | + | − | − | PRC |
| Pseudomonas mandelii PD8 | + | + | − | PRC |
| Pseudomonas grimontii PD9 | + | − | − | PRC |
| Pseudomonas grimontii PD10 | + | − | − | PRC |
| Pseudomonas lini PD11 | + | − | − | PRC |
| Sinorhizobium sp. strain PD12 | + | − | − | PRC |
| Pseudomonas mandelii PD13 | + | + | − | PRC |
| Pseudomonas sp. strain PD14 | + | − | − | PRC |
| Pseudomonas lini PD15 | + | − | − | PRC |
| Pseudomonas sp. strain PD16 | + | − | − | PRC |
| Pseudomonas migulae PD17 | + | + | − | PRC |
| Bosea sp. strain PD18 | + | − | + | PRC |
| Bosea sp. strain PD19 | + | − | + | PRC |
| Achromobacter sp. strain PD20 | + | − | − | PRC |
| Pseudomonas sp. strain PD21 | + | + | − | PRC |
| Pseudomonas sp. strain PD22 | + | + | − | PRC |
| Bosea sp. strain PD23 | + | − | + | PRC |
| Bosea sp. strain PD24 | + | − | + | PRC |
| Achromobacter sp. strain PD25 | + | − | − | PRC |
| Pseudomonas sp. strain PD26 | + | − | − | PRC |
| Achromobacter sp. strain PD27 | + | − | − | PRC |
| Pseudomonas lini PD28 | + | − | − | PRC |
| Ensifer adhaerens PD29 | + | − | + | PRC |
| Pseudomonas mandelii PD30 | + | + | − | PRC |
| Pseudomonas kilonensis PD31 | + | − | − | PRC |
a
+, visible band of expected size; −, no visible band; ATCC, American Type Culture Collection (Manassas, Virginia); Oxoid, Oxoid Quality Control organisms (Nepean, Ontario, Canada); UBC, University of British Columbia (Vancouver, British Columbia, Canada); DSMZ, Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (Braunschweig, Germany); USDA-ARS, United States Department of Agriculture-Agricultural Research Service (Beltsville, MD); PRC, Potato Research Centre (Fredericton, New Brunswick, Canada).