TABLE 2.
Primers/probes and conditions used for real-time PCR amplifications
| Primer/probe | Sequence (5′-3′) | Positiona | Concn (nM) | Reference |
|---|---|---|---|---|
| 1369F | CGG TGA ATA CGT TCY CGG | 1369-1386 | 900 | 33 |
| 1492R | GGW TAC CTT GTT ACG ACT | 1475-1492 | 900 | 33 |
| TM1389Fb | 6-FAM-CTT GTA CAC ACC GCC CGT C-TAMRAc | 1389-1407 | 300 | 33 |
| cnorBPF | CAT GGC GCT GAT AAC GGG | 738-755 | 900 | This study |
| cnorBPR | CTT IAC CAT GCT GAA GGC G | 870-888 | 900 | This study |
| cnorBBF | AIG TGG TCG AGA AGT GGC TCT A | 653-674 | 900 | This study |
| cnorBBR | TCT GIA CGG TGA AGA TCA CC | 810-829 | 900 | This study |
a
Numbering refers to the Escherichia coli sequence for 16S rRNA gene primers, the Pseudomonas fluorescens cnorB sequence (nucleotides 828 to 2255 of AF197467) for cnorBP primers, and the Bradyrhizobium japonicum sequence (nucleotides 757 to 2115 of AJ132911) for cnorBB primers.
b
The TaqMan detection system was used for 16S rRNA only.
c
FAM, 6-carboxyfluorescein; TAMRA, carboxytetramethylrhodamine.