TABLE 6.
CLAT detection of F+ DNA coliphage field isolates
| F+ DNA coliphage detection method | No. (%) of F+ DNA coliphage field isolatesc (n = 164)
|
No. (%) of false-positive results for F+ RNA coliphage field isolatesc (n = 132) | |
|---|---|---|---|
| True positive | False negative | ||
| CLATa | 161 (97.7)b | 3 (2.3) | 3 (1.8) |
| RNase neutralization assay | 164 (100) | 0 | 0 |
a
CLAT for F+ DNA coliphages used both M13 antiserum (1:8 antiserum dilution) and Fd antiserum (1:16 antiserum dilution).
b
No significant difference was detected between the proportions of truly positive F+ coliphage DNAs detected by the two methods (P value = 0.82).
c
Field isolates were plaque-purified enrichments of coliphages recovered from estuarine water and shellfish.