TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| Strains | ||
| S. enterica serovar Typhimurium | ||
| 12023 | WT | Lab collection |
| EE658 | SL1344 hilA::Tn5 lacZY | 1 |
| E. coli DH5α | General cloning strain | Invitrogen |
| S. flexneri | Virulence plasmid-cured | A. Blocker, Oxford, United Kingdom |
| Plasmids | ||
| p2795 | Core template vector | 10 |
| p3121 | luc template vector | This study |
| p3126 | phoA template vector | This study |
| p3138 | lacZ template vector | This study |
| p3141 | HaloTag template vector | This study |
| p3142 | CAT template vector | This study |
| p3143 | blaM template vector | This study |
| p3174 | GFPmut3 template vector | This study |
| p3176 | DsRed template vector | This study |
| pKD46 | Red-expressing vector | 4 |
| pCP20 | FLP-expressing vector | 4 |
| pFPV25.1 | Constitutive GFPmut3 expression | 3 |