TABLE 2.
Oligonucleotides used in this study
| Designation | Sequencea |
|---|---|
| SifA-Red-Luc-For | 5′-ATTATGTAGTCATTTTTACTCCAGTATAAGTGAGATTAATATGGAAGACGCCAAAAACATAA-3′ |
| SifA-Red-LacZ-For | 5′-CAGTATAAGTGAGATTAATATGCCGATTACTATAGGGAATCCCGTCGTTTTACAACGTCG-3′ |
| SifA-Red-Rep-Rev | 5′-ACCCTGAACGTGACGTCTGAGAAAGCGTCGTCTGATTTTAGTGTAGGCTGGAGCTGCTTC-3′ |
| SseJ200-Red-Luc-For | 5′-TTTATCATCTCCACACTTCTTAGGTAAAGAGATGCTTAATGAAGACGCCAAAAACATAA-3′ |
| SseJ-Red-GFPm-For | 5′-TTTGCTAAAGCGTGTTTAATAAAGTAAGGAGGACACTATGAGTAAAGGAGAAGAACTTTTC-3′ |
| SseJ-Red-LacZ-For | 5′-TTAATAAAGTAAGGAGGACACTATGCCATTGAGTGTTGGACCCGTCGTTTTACAACGTCG-3′ |
| SseJ-Red-Rep-Rev | 5′-CGGCACTATGATATTGAGCTGTGTTTTGCTCAAGGCGTACGTGTAGGCTGGAGCTGCTTC-3′ |
| SopE2-Red-Luc-For | 5′-TATAGAAAATATTGCGAATAAGTATCTTCAGAATGCCTCCGAAGACGCCAAAAACATAA-3′ |
| SopE2-Red-Rep-Rev | 5′-GGCAAACCAGCGCCAATGCAGGTGTCGTTACCGTGGCGACCCGTGTAGGCTGGAGCTGCTTC-3′ |
| MgtA-Red-LacZ-For | 5′-TGACTTCGGCGCGGAGGGATTACCTATGCTAAAAATCATTCCCGTCGTTTTACAACGTCG-3′ |
| MgtA-Red-Luc-For | 5′-GAATTTTCTGCGCCTGACTTCGGCGCGGAGGGATTACCTATGGAAGACGCCAAAAACATA-3′ |
| MgtA-Red-Rep-Rev | 5′-TTAAGCACGCTGGCGAATCCCCGACGAAAGTGTTTACTGCGTGTAGGCTGGAGCTGCTTC-3′ |
| EC-MgtA-Red-LacZ-For | 5′-TTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTCCCGTCGTTTTACAACGTCG-3′ |
| EC-MgtA-Red-Luc-For | 5′-CATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGGAAGACGCCAAAAACATA-3′ |
| EC-MgtA-Red-Rep-Rev | 5′-GCCGTAACGACGGCTATAGAACCCTTTCACCAACTGGGTCGTGTAGGCTGGAGCTGCTTC-3′ |
| GyrA-Red-SDo-Luc-For | 5′-GGAAAGCGATGACGACGTTGCGGATGACGCTGACGAGTAAAAACTGGAGACTGTCATGGAAGACGCCAAAAACAT-3′ |
| GyrA-Red-Rep-Rev | 5′-AAAGAAAAAGGGCCGGATATCCGGCCCTCGCACAGCAATACGTGTAGGCTGGAGCTGCTTC-3′ |
| Luc-Red-Check-For | 5′-GAGGAGTTGTGTTTGTGGAC-3′ |
| Luc-Red-Check-Rev | 5′-AGTAGTGACAAGTGTTGGCC-3′ |
| GFP-Red-Check-For | 5′-CAGACAACCATTACCTGTCC-3′ |
| GFP-Red-Check-Rev | 5′-AGGTAGTTTTCCAGTAGTGC-3′ |
| LacZ-Red-Check-Rev | 5′-TGTGAGCGAGTAACAACCCG-3′ |
a
Nucleotides complementary to the template plasmids are set in italics, and the portion containing the RBS is underlined.