TABLE 1.

No. of recognition sites per sequenced genomes and comparison of resistance to REase cleavage in different studies

REase	Recognized sequencea	No. of recognition sites per sequenced genomeb			% of strains resistant to cleavage (recognition site methylated)
		26695	J99	HPAG1	Present study (n = 51)	A (n = 122)c	B (n = 19)d	C (n = 16)e
BseRI	GAGGAG	149	141	128	100
EagI	CGGCCG	57	61	53	100
HhaI	GCGC	5,924	6,035	5,638	100			100
Hinp1I	GCGC	5,924	6,035	5,638	100
NaeI	GCCGGC	150	167	139	100
NgoMIV	GCCGGC	150	167	139	100
NlaIII	CATG	7,172	7,335	6,687	100	100	100	100
DpnI	GmATC	5,348	5,396	4,839	98
DpnII	GATC	5,348	5,396	4,839	98
BssHII	GCGCGC	282	303	266	96
AseI	ATTAAT	484	424	426	90
TacI	TCGA	303	336	275	88	86	63
HpaII	CCGG	1,731	1,808	1,654	82	67
HpyCH4V	TGCA	5,662	5,110	5,206	82	71
MspI	CCGG	1,731	1,808	1,654	82
HpyCH4IV	ACGT	282	500	278	65	74
HpyCH4III	ACNGT	557	587	503	55	51
DdeI	CTNAG	3,024	2,998	2,665	37	45	32	38
Hpy188I	TCNGA	1,396	1,244	1,209	37	21	84	13
FauI	CCCGC	1,657	1,637	1,535	24
HaeIII	GGCC	1,607	1,598	1,540	24	38	21	13
BstUI	CGCG	2,478	2,664	2,358	14
Hpy99I	CGWCG	247	267	244	14	62
Sau96I	GGNCC	837	865	803	14
AciI	CCGC	7,038	7,033	6,610	8
BssKI	CCNGG	1,385	1,435	1,289	8
ScrFI	CCNGG	1,385	1,435	1,289	8
DraI	TTTAAA	4,905	4,692	4,338	6
Fnu4HI	GCNGC	2,487	2,313	2,267	2
FokI	GGATG	1,353	1,328	1,235	0	2	16	6
Sau3AI	GATC	5,348	5,396	4,839	0

^aData from REBASE (38).

^bData from The Comprehensive Microbial Resource (34).

^cData from Takata et al. (43).

^dData from Ando et al. (2).

^eData from Xu et al. (48).