TABLE 2.
Observed changes in the methylation status of sequential isolates from the same patient
| Patient | Theoretical maximal no. of changesa | No. of observed changesa | Strains | Observed changed methylationb,c | Same methylation status? | Same profile with different typing methodsd?
|
|
|---|---|---|---|---|---|---|---|
| RAPD (% similarity) | PCR-RFLP (% similarity) | ||||||
| A | 168 (28 × 6) | 1 | P22.1 and P22.2 | None | Yes | Yes | Yes |
| P22.3 and P22.4 | None | Yes | Yes | Yes | |||
| P22.1/P22.2 and P22.3/P22.4 | +M.TacI | No | Yes | Yes | |||
| B | 168 (28 × 6) | 4 | P23.1 and P23.2 | M.HpyCH4III, M.DraI | No | Yes | Yes |
| P23.3 and P23.4 | None | Yes | Yes | Yes | |||
| P23.1 and P23.3/P23.4 | +M.HpyCH4III | No | No (93) | Yes | |||
| P23.2 and P23.3/P23.4 | −M.DraI | No | No (93) | Yes | |||
| C | 28 | 1 | P24.1 and P24.2 | +M.FauI | No | No (97) | Yes |
| D | 28 | 2 | P25.1 and P25.2 | +M.HpyCH4III, −M.Sau96I | No | No (90) | Yes |
| E | 56 (28 × 2) | 9 | P26.1 and P26.2 | −M.DdeI, +M.Hpy188I, −M.HpyCH4IV, −M.BssHII, −M.Sau96I | No | No (41) | No (55) |
| P26.2 and P26.3 | −M.HpyCH4V, +M.BssHII, −M.FauI, +M.Sau96I | No | NT | NT | |||
| F | 56 (28 × 2) | 6 | P27.1 and P27.2 | −M.HpyCH4IV, +M.HpyCH4V | No | NT | NT |
| P27.2 and P28.3 | +M.Hpy188I, −M.HpyCH4III, +M.HpyCH4IV, −M.HpyCH4V | No | NT | NT | |||
| G | 28 | 1 | P28.1 and P28.2 | +M.Hpy99I | No | NT | NT |
| H | 28 | 2 | P29.1 and P29.2 | +M.Hpy99I, +M.HpyCH4V | No | NT | NT |
| I | 28 | 5 | P30.1 and P30.2 | +M.MspI, +M.ScFI, +M.FauI, +M.TacI, +M.Sau96I | No | NT | NT |
| J | 28 | 1 | P31.1 and P31.2 | −M.Sau96I | No | NT | NT |
| K | 28 | 1 | P32.1 and P32.2 | M.AciI | No | Yes | Yes |
| Total | 644 | 33 | |||||
a
The MTase alteration represented 5.1% (33/644) of the results, and the maintenance of the expressed MTase was 94,9% among the sequential isolated strains.
b
Isoschizomers not included.
c
+, MTase acquisition; −, MTase loss; no plus or minus sign, impossible to determine if the MTase alteration was an acquisition or loss.
d
“Yes” alone represents 100% similarity. When strains do not have the same profile, the similarity percentage is shown. NT, not tested. Data are from reference 32.