TABLE 2.
Analysis of ORF products proposed to be involved in CMN production
| Predicted ORF product (no. of amino acids) | Homolog for VIO biosynthesis (no. of amino acids) | % Identity | Predicted function(s)a |
|---|---|---|---|
| CmnR (238) | VioR (263) | 49 | Transcriptional regulator (LuxR family) |
| CmnU (240) | CMN resistance; rRNA methyltransferase | ||
| CmnA (2,642) | VioA (2,123) | 53 | NRPS (X-A-PCP-C-A-PCP-C) |
| CmnB (370) | VioB (346) | 65 | l-2,3-DAP formation |
| CmnC (339) | VioC (358) | 62 | CAM formation |
| CmnD (374) | VioD (389) | 65 | CAM formation |
| CmnE (416) | VioE (447) | 63 | CMN efflux |
| CmnF (1,057) | VioF (1,073) | 56 | NRPS (A-PCP-C) |
| CmnG (953) | VioG (1,088) | 48 | NRPS (C-A-PCP-C/) |
| CmnH (249) | VioH (262) | 52 | Type II thioesterase |
| CmnI (549) | VioI (550) | 52 | NRPS (PCP-C) |
| CmnJ (384) | VioJ (390) | 62 | l-2,3-DAP α,β-desaturase |
| CmnK (333) | VioK (360) | 62 | l-2,3-DAP formation |
| CmnL (296) | VioL (308) | 65 | Carbamoyltransferase |
| CmnM (402) | VioM (457) | 45 | NRPS (C); β-Lys attachment |
| CmnN (63) | VioN (63) | 75 | Unknown function |
| CmnO (585) | VioO (610) | 52 | NRPS (A-PCP); β-Lys attachment |
| CmnP (449) | VioP (445) | 77 | β-Lys formation |
| Cph (282) | Vph (293) | 53 | CMN resistance; CMN phosphotransferase |
a
Abbreviations: A, adenylation domain; PCP, peptidyl carrier protein domain; C, condensation domain; X, domain of unknown function; C/, modified condensation domain; CAM, 2S,3R-capreomycidine.