TABLE 3.
CMN or VIO NRPS componenta | Specificity codeb | Amino acid proposed to be activatedc |
---|---|---|
CmnF | D A Q S L A V V | l-2,3-DAP |
VioF | D A Q S L A I V | l-2,3-DAP |
CmnA, A1 | D V Y H F S L V | l-Ser (or l-Ala) |
VioA, A1 | D V Y H F S L V | l-Ser |
CmnA, A2 | D V R S L S M V | l-2,3-DAP |
VioA, A2 | D V R H M S M V | l-Ser |
CmnG | D P Q D I G I V | CAM |
VioG | D P Q D V G I G | CAM |
CmnO | D T E D V G T M | β-Lys |
VioO | D T E D V G V G | β-Lys |
NRPS components are grouped according to homology (e.g., VioF is the homolog of CmnF). CmnA and VioA each contain more than one A domain. The first A domain is noted as A1, and the second is noted as A2.
Specificity code as described in references 6 and 30. The alignment program to identify the substrate specificity code is available at http://www.tigr.org/jravel/nrps.
This proposal is based on the results of the A domain substrate specificity code and the chemical structures of CMN and VIO. CAM, 2S,3R-capreomycidine.