TABLE 4.
Changes in CMN and kanamycin sensitivity upon expression of cmnUa
| Strain (relevant genotype)b | MIC (μg/ml) of:
|
|
|---|---|---|
| CMN | Kanamycin | |
| E. coli DH5α/pBAD33/pLJ102 (cmnU tlyA) | 50 | 12 |
| E. coli DH5α/pBAD33-cmnU/pLJ102 (cmnU+tlyA) | 100 | 96 |
| E. coli DH5α/pBAD33/pSJ101 (cmnU tlyA+) | 25 | 12 |
| E. coli DH5α/pBAD33-cmnU/pSJ101 (cmnU+tlyA+) | 50 | 24 |
| S. lividans 1326/pSE34 (cmnU) | 200 | 6 |
| S. lividans 1326/pSE34-cmnU (cmnU+) | >1,600 | >1,600 |
a
For E. coli strains, the MIC was defined as the lowest antibiotic concentration at which the optical density at 600 nm after 16 h of incubation at 37°C in LB medium was <0.1. For S. lividans 1326 strains, the MIC was defined as the lowest antibiotic concentration at which no growth on ISP2 plates was observed after 2 days of incubation at 30°C. MICs reported are from three separate experiments. See Materials and Methods for details.
b
Gene designations lacking a superscript plus sign represent genes not carried on plasmids.