FIG. 2.

16S rRNA gene-based phylogenetic tree for selected isolates of Chloroflexi. The tree includes clones sequenced from biosolids (B) and soil (S), downwind aerosols (D), and selected type strains isolated from wastewater environments. The tree is based on the neighbor-joining method; the bar indicates 10% estimated sequence divergence. Closed circles represent >50% bootstrap values, and open circles represent <50% bootstrap values. The tree is rooted using E. coli (AM184252) as the out-group (not shown).