FIG. 4.
16S rRNA gene-based phylogenetic tree sequences from cultured sulfite-reducing Clostridia isolated from biosolids (B) and soil (S) and selected type strains. Branch I contains C. bifermentans sequences from biosolids and from soils, and branch II contains C. subterminale sequences from soil. The tree is based on the neighbor-joining method. Closed circles represent >50% bootstrap values, and open circles represent <50% bootstrap values. The tree is rooted using Geoglobus ahangari (AF220165) as the out-group (not shown).