TABLE 1.
PCR primers used in this study
| Primer name | Primer target group | Primer sequence (5′→3′) | Amplicon size (bp) | Reference |
|---|---|---|---|---|
| 27F | Domain Bacteria | AGAGTTTGATCCTGGCTCAG | 900 | 22 |
| 907R | Universal | CCGTCAATTCCTTTRAGTTT | 22 | |
| 515F | Universal | GTGCCAGCMGCCGCGG | 899 | 22 |
| 1392R | Universal | ACGGGCGGTGTGT[A:G]C | 22 | |
| A21F | Domain Archaea | TTCCGGTTGATCC[C:T]GCCGGA | 1,351 | 9 |
| 1392R | Universal | ACGGGCGGTGTGT[A:G]C | 22 | |
| MB301F | Methanobacteriales | CTTGTCTCAGGTTCCATCTCCG | 608 | 41 |
| AR912R | Domain Archaea | CTCCCCCGCCAATTCCTTTA | 24 | |
| CH608 | Chloroflexi sp. | CCAAGCTTGAGGATGGTAGA | 516 | This study |
| CH1124 | Chloroflexi sp. | GACTTAATATCGGCAGTACCGTG | This study |