FIG. 7.

Effect of tTG-deamidated PT digests of proteins extracted from CAD (columns 1, 2, and 3) and S2E12 (columns 4, 5, and 6) on IFN-γ production by iTCLs. Columns: Cn, negative control (medium alone); 1 and 4, albumins and globulins; 2 and 5, gliadins; 3 and 6, glutenins; Sg, PT digest of gliadins from cv. Sagittario. The IFN-γ level in 48-h cell culture supernatant was measured by a sandwich ELISA. S2E12 ingredients are described in Materials and Methods. Data are the means ± standard deviations from two independent fermentations each analyzed twice.