TABLE 2.
Presence and activities of GshR, gshR, and cysteine-γ-lyase in sourdough lactobacilli
| Strain | GshR activity (nmol min−1 mg−1)a | Presence of gshRb | Presence of cystathionine-γ-lyase activity | Presence of cystathionine-γ-lyasec |
|---|---|---|---|---|
| L. sanfranciscensis DSM20451 | 45 | + | − | −d |
| L. sanfranciscensis DSM20451ΔgshR | 14 | − | − | ND |
| L. reuteri BR11 | − | − | + | +e |
| L. reuteri BR11ΔcyuC | − | − | + | ND |
| L. pontis LTH2587 | − | − | + | +d |
a
GshR activity in crude cellular extract. −, absence of GshR activity in crude cellular extracts after separation by SDS-PAGE.
b
Detection of gshR was performed by using the degenerate primers deg/gshRV and deg/gshRR.
c
Detection of cystathionine-γ-lyase was performed by PCR.
d
See reference 45.
e
See reference 40.