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Enrichment for Salmonella serotype Typhimurium mutants expressing PefA by using the SIMPLE method. (A) Schematic illustrates the principle of the SIMPLE method. (B) Western blotting analysis of four pools, each originally consisting of approximately 1,500 independent mutants, after two rounds of enrichment by the SIMPLE method. From each pool, 24 colonies were randomly selected and grown statically in LB broth at pH 7, and a mixture of 12 mutants was loaded per lane.
