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Positions of T-POP insertions identified by the SIMPLE method for Salmonella serotype Typhimurium mutants expressing PefA. The open arrow indicates the position and orientation of the hns open reading frame. The T-POP insertion sites in mutants SPN66, SPN67, SPN68, and SPN69 are indicated. Arrows indicate the orientation of the tetracycline-inducible tetA promoter of T-POP.
