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. 2007 May 25;73(14):4455–4462. doi: 10.1128/AEM.00148-07

FIG. 6.

FIG. 6.

Enrichment by immunomagnetic separation for Salmonella serotype Typhimurium hns(+281)::T-POP mutants expressing PefA on their surfaces. Detection of fimbrial filaments composed of PefA on the surface of (A) Salmonella serotype Typhimurium SR11 ΔfimAICDHF::Km hns(+281)::T-POP (SPN68) or (B) Salmonella serotype Typhimurium SR11 hns(+281)::T-POP (SPN84) by immunogold labeling. Bacteria were grown statically in LB broth at pH 7 without a yellow pipette tip. (C) n-fold enrichment is shown for SPN68 [Δfim::Km hns(+281)::T-POP; left panel] or SPN84 [hns(+281)::T-POP; right panel] accomplished by a single round of immunomagnetic separation with particles coated with anti-PefA serum (+) or not coated with serum (−). Magnetic particles were incubated with the indicated mixtures of strains SPN22 and SPN68 (left panel) or SR11 and SPN84 (right panel). Data are shown as geometric means ± standard deviations of three independent experiments. Statistical significance (P value) of differences between treatment groups (shown in brackets) is indicated above the bars.