TABLE 1.
Bacterial strains used in this study
| Bacterial strain(s) | Genotype | Source or reference |
|---|---|---|
| E. coli | ||
| DH5α MCR | F′ mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 phoA supE44 λ−thi-1 gyrA96 relA1 | Gibco BRL |
| ORN172 Nalr | Nalrthr-1 leuB thi-1 Δ(argF-lac)U169 malA1 xyl-7 ara-13 mtl-2 gal-6 rpsL tonA2 supE44 ΔfimBEACDFGH::Km | 31 |
| Salmonella serotype Typhimurium | ||
| IR715 | Wild-type isolate ATCC 14028, Nalr | 27 |
| EHW2 | IR715 ΔfimAICDHF::Km | 30 |
| LT2 | Laboratory strain | 15 |
| TH3923 | LT2 pJS28(Carbr P22-9+)/F′114ts Lac+zzf-20::Tn10[tetA::MudP](Tcs) zzf-3823::Tn10dTc[del-25](T-POP)/leuA414 hsdSB Fels2− | K. Hughes |
| TH4881 | LT2 pNK2880(CarbR, Tn10 tnpA[ats-1 ats-2])/fliC5050::MudJ fljB5001::MudCm | 22 |
| SL1344(T-POP) | Wild-type isolate SL1344 fimZ2::T-POP | 5 |
| SR11 | Xylose-fermenting mutant of wild-type isolate BA2 | 24 |
| AJB3 | SR11 Nalr | 3 |
| ADH19 | SR11 NalrΔpefBACD::Km | 13 |
| SPN22 | SR11 NalrΔfimAICDHF::Km | This study |
| SPN23 | LT2 ΔfimAICDHF::Km | This study |
| SPN66 and SPN67 | SR11 NalrΔfimAICDHF::Km hns(−195)::T-POP | This study |
| SPN68 | SR11 NalrΔfimAICDHF::Km hns(+281)::T-POP | This study |
| SPN69 | SR11 NalrΔfimAICDHF::Km hns(−224)::T-POP | This study |
| SPN71 | SR11 fimZ2::T-POP | This study |
| SPN74 | SR11 Nalr ΔfimAICDHF::Km fimZ2::T-POP | This study |
| SPN81 | SR11 hns(−195)::T-POP | This study |
| SPN84 | SR11 hns(+281)::T-POP | This study |