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Confocal microscopic detection of GFP expression in C. fetus subsp. venerealis ATCC 19438. Vector pRYEL1 was mobilized to C. fetus subsp. venerealis, and Cmr transconjugants were selected. Bacteria were scraped from a 48-h CBA plate, suspended in PBS, and observed by confocal microscopy for differential interference contrast (A) and green fluorescence (B). Merged images are shown in panel C.
