TABLE 3.
Plasmids used in this study
| Plasmid (size in kb) | Relevant features | Reference or source |
|---|---|---|
| pRYGG1 (7.32) | sapA108 promoter (PsapA) in the pRY111 MCS | This study |
| pRYGG2 (9.24) | Fusion of PsapA to 1.9-kb tetO coding sequence in pRYGG1 | This study |
| pRYSS1 (8.15) | Φ(PsapA-aphA-3), 0.8-kb aphA-3 inserted in pRYGG1 | This study |
| pRYVL1 (8.15) | Φ(PsapA-aphA-3) in pRYGG1, PstI and EcoRI linker inserted between PsapA and aphA-3 | This study |
| pRYVL2 (10.49) | Φ(PsapA-virD4-aphA-3), 2.2-kb gene inserted between PsapA and aphA-3 of pRYVL1 | This study |
| pRYMJ1 (12.77) | Φ(PsapA-C. fetus subsp. venerealis operon), 5.47-kb coding sequence inserted in pRYGG1 | This study |
| pRYEL1 (8.06) | Φ(PsapA-gfp) in pRYGG1 | This study |
| pCFV108 (3.72) | Cryptic plasmid isolated from C. fetus subsp. venerealis 4111/108 | This study |
| pSK108-1 (6.17) | 3,207 bp of pCFV108 including mobA, repE and iterons in pBluescriptKSII(−) | This study |
| pRYSK1 (8.10) | C. coli oriV of pRYSS1 replaced by repE and iterons of pCFV108 | This study |
| pRYSK2 (6.58) | repE deletion of pRYSK1, pCFV108 iterons alone | This study |
| pRYSK3 (5.61) | pCFV108 iterons, Φ(PsapA-aphA-3) in pBlue-oriT | This study |
| pRYSK4 (6.72) | Φ(PsapA-tetO), aphA-3 of pRYSK3 replaced by tetO | This study |
| pRYSK5 (9.33) | Φ(PsapA-gyrA-tetO), 2.6-kb gyrA inserted between PsapA and tetO of pRYSK4 | This study |
| pBlue-oriT (3.72) | mobIncP of pILL570 in the pBluescriptKSII(−) MCS | This study |
| pBluescriptIIKS(−) | bla lacZ | Stratagene |
| pATJ200 | Φ(PCff-sapA-tetO) PCc-aphA-3 mobIncPoriVpIP1455oriVpBR322, source of tetO | 14 |
| pRY107 | PCc-aphA-3 mobIncPoriVpIP1455oriVpBR322 | 65 |
| pRY111 | PCc-cat mobIncPoriVpIP1455oriVpBR322, source of cat | 65 |
| pILL570 | Source of mobIncP | 30 |
| pILL600 | Source of aphA-3 | 28 |
| pWM1007 | Source of gfp | 36 |