FIG. 5.

Effects on proteins in E. coli O157:H7 ATCC 43895 cells after addition of carvacrol to cells in exponential phase. (A) Representative Western blots of HSP60 (GroEL), HSP70 (DnaK), and flagellin after treatment with increasing concentrations of carvacrol. Cells were grown in MHB, resuspended in MHB containing carvacrol, and incubated for a further 3 h. (B) Relative density results from three independent experiments, quantified by densitometric analysis of the Western blots represented in panel A. The bars represent the means ± SD of the results. Treatment results that are significantly different from each other are indicated with different letters (P < 0.05).