FIG. 8.

Effects on proteins in E. coli O157:H7 ATCC 43895 cells after addition of p-cymene to cells in exponential phase. (A) Representative Western blot of HSP60 (GroEL), HSP70 (DnaK), and flagellin synthesis after treatment with increasing concentrations of p-cymene. The cells were grown in MHB, resuspended in MHB containing p-cymene, and incubated for a further 3 h. (B) Relative density results from two independent experiments, quantified by densitometric analysis of the Western blots represented in panel A. The bars represent the means ± SD of the results.