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Most relevant properties of the library of probes generated in the first step of our microarray design. (A) Distribution of the numbers of probes per target. An average of 32 different probes per target was obtained. (B) Tm distribution. As different programs use different methods and/or sets of constants to calculate the Tm of a probe, all of the Tms were recalculated using Hybrid 2.5 (16) as described in reference 32. (C) G+C content distribution.
