TABLE 2.
Gene expression data in microarray for the 19 selected probe sets
| Homo sapiens gene or gene product | Probe set | Accession no. | Mean change (n-fold) vs. mock (n = 2)a
|
||
|---|---|---|---|---|---|
| 24 h Cpn | 24 h Cpn + IFN-γ | 96 h Cpn + IFN-γ | |||
| 2′-5′oligoadenylate synthetase-like (OASL) | 205660_at | NM_198213 | NC | I (5.2/5.5) | I (1.9/3.7) |
| 2′-5′oligoadenylate synthetase-like (OASL) | 210797_s_at | NM_198213 | NC | I (5.6/6.2) | I (2.2/3.0) |
| Adlican | 209596_at | AF245505.1 | D (1.7/1.8) | D (NC/1.5) | D (3.5/3.2) |
| BCL2 adenovirus E1B 19-kDa interacting protein 3 (BNIP3) | 221478_at | NM_004052 | D (2.6/2.1) | D (2.2/1.8) | D (2.8/2.6) |
| BCL2 adenovirus E1B 19-kDa interacting protein 3 (BNIP3) | 201848_s_at | NM_004052 | D (3.4/1.5) | NC | D (3.0/3.3) |
| Carbonic anhydrase IX (CA9) | 205199_at | NM_001216 | D (2.3/2.2) | D (NC/1.8) | D (15.6/7.9) |
| Cystathionase (cystathionine gamma-lyase) (CTH) | 206085_s_at | NM_001902 | D (1.2/1.1) | D (4.5/2.9) | D (NC/1.8) |
| Cystathionase (cystathionine gamma-lyase) (CTH) | 217127_at | NM_001902 | D (2.1/1.3) | D (5.7/3.9) | D (NC/1.3) |
| Cysteine-rich, angiogenic inducer, 61 (CYR61) | 210764_s_at | AF003114.1 | I (5.7/4.1) | I (2.5/1.8) | I (1.6/NC) |
| Dapper homolog 1 (DACT1) | 219179_at | NM_016651 | NC | D (1.9/2.7) | D (2.9/2.5) |
| Dickkopf (Xenopus laevis) homolog 1 (DKK1) | 204602_at | NM_012242 | I (4.0/4.2) | I (2.5/2.4) | I (3.5/4.1) |
| Hairy/enhancer-of-split related with YRPW motif 1 (HEY1) | 44783_s_at | NM_012258 | D (4.2/3.7) | D (4.9/5.7) | D (1.4/NC) |
| Heat shock 70-kDa protein 1A (HSPA1A) | 200799_at | NM_005345 | NC | I (10.3/7.3) | D (2.0/1.7) |
| Heat shock 70-kDa protein 1A (HSPA1A) | 200800_s_at | NM_005345 | NC | I (14.4/11.8) | D (1.6/1.7) |
| Heat shock 70-kDa protein 1B (HSPA1B) | 202581_at | NM_005346 | D (NC/1.5) | I (13.6/9.0) | D (NC/1.7) |
| Insulin-induced gene 1 (INSIG1) | 201626_at | NM_005542 | D (3.9/3.2) | D (2.3/1.9) | D (1.8/3.5) |
| Insulin-induced gene 1 (INSIG1) | 201627_s_at | NM_005542 | D (3.0/3.8) | D (2.6/2.3) | NC |
| Interferon-induced, hepatitis C-associated microtubular aggregate protein (44 kDa) (MTAP44 or IFI44) | 214453_s_at | NM_006417 | I (1.7/NC) | I (5.4/5.0) | I (2.4/3.8) |
| Interferon-induced, hepatitis C-associated microtubular aggregate protein (44 kDa) (MTAP44 or IFI44) | 214059_at | NM_006417 | NC | I (3.3/4.4) | NC |
| Keratin 17 (KRT17) | 212236_x_at | NM_000422 | I (2.1/2.0) | I (1.9/2.0) | D (3.5/3.9) |
| Keratin 17 (KRT17) | 205157_s_at | NM_000422 | I (2.4/2.0) | I (1.6/.03) | D (3.0/5.4) |
| Lysyl oxidase (LOX) gene | 215446_s_at | L16895 | D (2.1/2.3) | D (2.5/2.1) | D (4.2/3.6) |
| N-myc downstream regulated (NDRG1) | 200632_s_at | NM_006096 | D (4.1/2.2) | D (3.0/2.1) | D (7.1/4.1) |
| Prostaglandin E receptor 4 (subtype EP4) (PTGER4) | 204897_at | NM_000958 | I (2.2/2.0) | I (2.4/2.3) | I (2.8/3.9) |
| Retinoic acid induced 3 (RAI3) | 203108_at | NM_003979 | I (3.8/2.7) | I (2.8/3.4) | I (2.0/1.5) |
| Serum-inducible kinase (SNK) or polo-like kinase 2 (PLK2) | 201939_at | NM_006622 | I (2.4/4.2) | NC | I (3.7/4.8) |
Altered gene expression data for selected Affymetrix HG-U133A probe sets. All 19 genes were selected for real-time two-step RT-PCR analysis. Expression changes between two arrays are designated “change (n-fold)” (treatment versus mock treatment at the corresponding time point). Depicted are the changes (D, decrease; I, increase; NC, no change) in two independent microarray experiments of C. pneumoniae-infected HeLa-cells (24 h Cpn = productive infection) in the IFN-γ persistence model (24 h and 96 h Cpn + IFN-γ). All selected genes had signal log ratios greater than 1.32 or lower than −1.32 (α1 = 0.05; α2 = 0.065; τ = 0.015) under at least one experimental condition and present calls in both samples.