TABLE 1.
Primers used in this study
| Gene or primer | Forward primer sequence | Reverse primer sequence | Probe sequence | Sequence | Description |
|---|---|---|---|---|---|
| Primers for amplifying coding sequence | |||||
| BBA64 | TTGAAGGATAACATTTTGAAAAAT | CTGAATTGGAGCAAGAATATTGGT | |||
| BBA65 | TTGAATAAAATAAAATTATCAATA | ATTATTATTAAATTTAAATAATGT | |||
| BBA66 | GTATTTTGAAAATCAAACCATTAA | CATTATACTAATGTATGCTTCAAG | |||
| BBA68 | TTGAAAAAAGCCAAACTAAATATA | GTAAAAGGCAGGTTTTAAAGTATC | |||
| BBA69 | TTGAAAAAAGCCAAACTAAATATA | ATAAAAGGCAGATTGTAAAGAATC | |||
| BBA70 | ATGGCTCCAGAAGTAAACAGCTAC | TTTTATATTAGTCTCTTTTTCAAT | |||
| BBA71 | AATGAATAAATTAAAAGAAATTCT | GTTTTGTTTAAATTCAACACTGTA | |||
| BBA73 | TTGAAAAGAAACAAAATTTGGAAA | GTAGTGTATGTGGTCACAACAGGT | |||
| BBI36/38 | TTGAAAAACTTTAAATTAAATACT | ATCAGCTTGATCTAGTAGATAAAG | |||
| BBI39/J41 | TTGAAAAACCTTAAATTAAATATT | ATCAGCTTGGTTTAGTAGATAAAG | |||
| vlsE | AGTAGTACGACGGGGAAACCAG | ACTTTGCGAACTGCAGACT (41) | |||
| TaqMan primers | |||||
| flaB | TCTTTTCTCTGGTGAGGGAGCT | TCCTTCCTGTTGAACACCCTCT | AAACTGCTCAGGCTGCACCGGTTC | ||
| BBA64 | AGCCCGCAGCTGGAAAA | AGCTTTGCAACTTCAGGATCTAATATT | AATCCCAACGCTAATGCCAACAATGCT | ||
| BBA65 | GCATGTTTGAACTGCTTAATGTTTACA | TGCGAGGCTTGAGTTCATTTT | TCCACAAATGGCACAAGGCTTCA | ||
| BBA66 | GCAAGAGCTGCATCACTAACAAA | TGTTGGCAGCCCGCTATT | TCAAGCCGTTACAACCGTACCCG | ||
| Primers for cloning into expression vector | |||||
| bba64.3 TR | TGCTCTCTGGAAGTCAAAGACAGC | Forward BBA64 truncated at Cys28 | |||
| bba64.2 FL+H3 | AAATTTAAGCTTCTGAATTGGAGCAAGAAT | Reverse BBA64 + HindIII site | |||
| bba65.1 FL+BamHI | AAATTTGGATCCTTGAATAAAATAAAATTATCAa | Forward full-length BBA65 + BamHI site | |||
| bba65.2 FL | ATTAAATTTAAATAATGTGTC | Reverse full-length BBA65 | |||
| bba66.3 TR | TGCACGATTGATGCCAATCTAAACG | Forward BBA66 truncated at Cys19 | |||
| bba66.2 FL+Xba | AAATTTTCTAGACATTATACTAATGTATGCa | Reverse BBA66 + XbaI site | |||
| malE forward | GGTCGTCAGACTGTCGATGAAGCC | Forward PCR primer for malE |
a
The restriction site is underlined.