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Immunoglobulin responses to the HC50 domain of BoNT/A. The polypeptide was administered to mice (n = 10) as an i.n. vaccine. Two weeks after the second booster, the dilution titers for IgG (▵) and for IgA (□) in serum were determined. The data show that mucosal vaccination evoked circulating titers of both antibodies.
